Abstract: Objective To investigate the relationship between DNA repair factor BRCA2 and epidermal growth factor inhibitor resistance in diffuse large B cell lymphoma. Methods Single cell cloning experiment was used to screen inhibitors of epidermal growth factor resistant cells. Western blot experiment was used to detect the expression of BRCA2 molecules in the resistant cells. RNA interference was used to specifically downregulate the expression of drug resistance BRCA2 cells. Proliferation and apoptosis of cells on BRCA2 expression and cell apoptosis and resistance to wash it well with new relationship between the ability of self. Results The successful selection of diffuse large B cell lymphoma cells resistant to inhibitors of epidermal growth factor. Western blot experiment, diffuse large B cell lymphoma epidermal growth factor inhibitor resistant cells with high expression of BRCA2; to explore the expression of BRCA2 and diffuse large B cell lymphoma by sub epidermal growth inhibitor resistance between the cell clone formation experiment showed that compared to the normal the number of clones resistant cells, down-regulation of BRCA2 cells 6 orifice hole single cell clone greater than 20 cells decreased 5 times; and the apoptosis of Tunel cells showed the resistance compared to normal cells, optical fluorescence microscopy observation of BRCA2 apoptosis ratio increased 6 times. Conclusions In this study, for the first time, we found that the expression of BRCA2 was highly expressed in the epidermal growth factor receptor (BRCA2) in diffuse large B cell lymphoma, and the down-regulation of the drug resistance in the resistant cells could promote the cell apoptosis and self-renewal ability. The relationship between DDR (DNA damage response) and EGFR was found by using the DNA damage response molecule as the breakthrough point, in order to provide more theoretical basis for the clinical treatment of diffuse large B cell lymphoma in the future.