胞质多聚腺苷酸化元件结合蛋白4对胶质瘤细胞生长的影响

范小明; 周佳

doi:10.16766/j.cnki.issn.1674-4152.000054

胞质多聚腺苷酸化元件结合蛋白4对胶质瘤细胞生长的影响

doi: 10.16766/j.cnki.issn.1674-4152.000054

范小明^1, ,,
周佳²

1. 金华市中医医院外三科, 浙江金华 321017;
2. 浙江省人民医院神经外科, 浙江杭州 310003

基金项目:

2014年浙江省医药卫生一般研究计划项目(2014-KYB-034)

详细信息

通讯作者:
范小明,E-mail:13566771770@163.com

中图分类号: R739.41;R329.28
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出版历程
- 收稿日期: 2017-03-17

Effects of cytoplasmic polyadenylation-element binding protein-4 on the growth of glioma cells

FAN Xiao-ming^{1
, ,},
ZHOU Jia²

Department of Surgery, Jinhua City Hospital of Traditional Chinese Medicine, Jinhua, Zhejiang 321017, China

摘要

摘要: 目的探讨胞质多聚腺苷酸化元件结合蛋白4(cytoplasmic polyadenylation-element-binding protein,CPEB4)在脑胶质瘤组织中的表达及其对胶质瘤细胞生长的影响。方法测定脑胶质瘤组织中CPEB4蛋白表达、脑胶质瘤组织和细胞中CPEB4 mRNA水平、细胞中CPEB4蛋白、PIK3CA蛋白、SMAD3蛋白的表达、细胞凋亡、细胞周期、细胞增殖和细胞的侵袭能力。结果低级别脑组织和高级别脑组织中CPEB4蛋白阳性率高于正常脑组织(P<0.05),高级别脑组织中CPEB4蛋白阳性率高于低级别脑组织(P<0.05);低级别脑组织和高级别脑组织中CPEB4蛋白水平高于正常脑组织(P<0.05),高级别脑组织中CPEB4蛋白水平高于低级别脑组织(P<0.05);SiRNA-CPEB4组细胞CPEB4蛋白表达量低于阴性对照组和空白对照组(均P<0.05);SiRNA-CPEB4组早期细胞凋亡率明显高于阴性对照组和空白对照组(均P<0.05);SiRNA-CPEB4组G₁期细胞比例高于阴性对照组和空白对照组(均P<0.05),SiRNA-CPEB4组S期细胞比例低于阴性对照组和空白对照组(均P<0.05);SiRNA-CPEB4组第3天、第7天细胞OD值均低于阴性对照组和空白对照组(P<0.05);SiRNA-CPEB4组侵袭细胞数低于阴性对照组和空白对照组;SiRNA-CPEB4组细胞PIK3CA蛋白、SMAD3蛋白表达量均低于阴性对照组和空白对照组(均P<0.05)。结论脑胶质瘤组织中CPEB4水平升高,CPEB4影响脑胶质瘤细胞的凋亡、细胞周期、增殖和侵袭,其机制可能和PIK3CA、SMAD3有关。
- 胞质多聚腺苷酸化元件结合蛋白4 /
- 脑胶质瘤 /
- 凋亡 /
- 增殖 /
- 侵袭
Abstract: Objective To investigate the expression of Cytoplasmic polyadenylation-element-binding protein 4(CPEB4) in glioma and its effect on glioma cell growth. Methods The expression of CPEB4 protein in glioma tissues, the expression of CPEB4 mRNA in brain glioma tissues and cells, the expression of CPEB4 protein, PIK3CA protein and SMAD3 protein, cell apoptosis, cell cycle, cell proliferation and the invasion ability of cells were detected. Results The positive rate of CPEB4 protein in low-grade brain tissue and high-level brain tissue were higher than that in normal brain tissue(P<0.05). The positive rate of CPEB4 protein in high-level brain tissue was higher than that in low-grade brain tissue(P<0.05). The CPEB4 protein levels in low-level brain tissue and high-level brain tissue were higher than that in normal brain tissue(P<0.05). The level of CPEB4 protein in high-level brain tissue was higher than that in low-grade brain tissue(P<0.05). The expression of CPEB4 protein in SiRNA-CPEB4 group was lower than that in negative control group and blank control group(P<0.05). The apoptotic rate in SiRNA-CPEB4 group was significantly higher than that in negative control group and blank control group(P<0.05). The percentage of G₁ phase cells in SiRNA-CPEB4 group was higher than that in negative control group and blank control group(P<0.05). The percentage of S phase cells in SiRNA-CPEB4 group was lower than that in negative control group and blank control group(P<0.05). The OD value at 3 days and 7 days in SiRNA-CPEB4 group were lower than that in negative control group and blank control group(P<0.05). The number of invasion cells in SiRNA-CPEB4 group was lower than that in negative control group and blank control group(P<0.05). The expression levels of PIK3CA and SMAD3 in SiRNA-CPEB4 group were significantly lower than those in negative control group and blank control group(P<0.05). Conclusion The level of CPEB4 in brain glioma is increased. CPEB4 could affect the apoptosis, cell cycle, proliferation and invasion of glioma cells. The mechanism may be related to PIK3CA and SMAD3.
- Cytoplasmic polyadenylation component-binding protein 4 /
- Glioma /
- Apoptosis /
- Proliferation /
- Invasion