Abstract: Objective To evaluate the health status of employees among different types in the high-tech enterprises and to understand the risk factors of health.To explore effective intervention for different types of employees after evaluating the difference between the intervention group and the control group. Methods The investigations were conducted twice among the employees of two high-tech institutes in Shanghai Caohejing Development Zone respectively. A series of targeted health education and health services were carried out in the intervention group. A total of 1 022 effective questionnaires were collected in the baseline survey conducted in 2012. A total of 1 113 effective questionnaires were collected in the final survey in 2014. Results After the intervention, the mental health of productive staffs was improved significantly. Some work related factors and health behavior were also improved significantly(P<0.05). The self-rated health and mental health of non-productive employees were improved significantly after the intervention(P<0.05). However, there was no significant difference in work related factors and health behavior. Most of work related factors and health behaviors appeared even worse. For the productive employees in control group, there was no significant difference in self-rated health, mental health and health behaviors. However, some work related factors have been improved. For the non-productive employees in control group, there was no difference in self-rated health, mental health, work related factors and health behavior(P>0.05). Conclusion The intervention effects differ by work types. Considering the characteristics of employees, more targeted health promotion should be developed.