Abstract: Objective To investigate the knowledge and skills of medical staffs in primary hospital on cardiopulmonary resuscitation, and provide basis for the promotion of CPR and improvement of CPR training in local hospitals. Methods A questionnaire study from August, 2013 to August, 2016 to assess the CPR knowledge skill of medical staffs from primary hospitals in three cities (Weifang, Zibo and Dezhou) was performed based on 2010 American Heart Association Guidelines for CPR and ECC. The data samples were collected by random sampling method among the medical staffs to process statistical analysis. Results The knowledge and skills of medical staffs in primary hospital on CPR:① The high requirement of primary medical staff on cardiopulmonary resuscitation theory, anatomical structure of human heart and lungs, recognition of sudden cardiac arrest, the steps of manual CPR, and the theory knowledge of primary life support and advanced life support. ② The high requirement for chest compression, artificial respiration, open the airway, automatic defibrillation, tracheal intubation, ring membrane puncture and tracheotomy. ③ Primary medical staff were lacking of cardiopulmonary resuscitation knowledge and skills. Conclusion The mastering of CPR among primary hospital staffs is relatively weak. They lack of understanding of CPR. But clinical work is still highly demanding for CPR. The hospital training based on different education background, work experience and titles can effectively improve the first aid knowledge and skills on cardiopulmonary resuscitation of the primary hospital medical staffs.