Abstract: Objective To explore the nursing features, nursing cooperation and matters needing attention of patients with 25G+ minimally invasive closed vitrectomy combined with 2 mm micro incision phacoemulsification and to prepare for standardized nursing of minimally invasive combined anterior and posterior operation. Methods Nursing care includes 67 cases of 25G+ minimally invasive closed vitrectomy combined with 2 mm incision phacoemulsification surgery from July, 2013 to March, 2016, Zhejiang Department of Ophthalmology, Lishui People's Hospital. In addition to the routine care of eye surgery, we should particular pay attention to the frequency of cutting speed, referring to the soft and hard wall of the eye wall, the size of the ultrasound energy, and the level of negative pressure with phacoemulsification and vitrectomy. Minimally invasive surgery of anterior with posterior segment has a special link to the timely adjustment of phaco machine and vitrectomy machine parameters according to the needs, to ensure the normal use of instruments and equipment. Liquid effusion box should not be too full, injection needle injector should be closely connected with silicone oil injection, uniform, slow and sustained action, so as to avoid the omentum under the gas accumulation. Results In the eyes of 67 eyes who received minimally invasive combined anterior and posterior surgery, 67 eyes had different degrees of vision improvement at the last follow-up, and 65 of them had visual acuity increased by 2 lines or more. No obvious corneal edema and corneal endothelial decompensation occurred after surgery. Besides, 9 eyes of diabetic traction retinal detachment were added after surgery. Conclusion Special characteristics of nursing were found in 25G+ minimally invasive vitrectomy combined with 2 mm incision phacoemulsification. Close cooperation was made during operation according to the operation process, the need for timely adjustment of phaco machine and vitrectomy machine mode as well as parameters, to ensure the normal use of instruments and equipment, and more changes in the operation difficulty, deeply familiar with equipment performance and skills, and close cooperation, to ensure the success of the 25G+ minimally invasive closed vitrectomy combined with 2 mm micro incision phacoemulsification.