北京儿童医院2013—2017年住院儿童肺炎链球菌分离株的血清型分析

刘莹; 董方; 史伟; 刘钢; 钱素云; 徐保平; 王青; 孟庆红; 申昆玲; 姚开虎

doi:10.16766/j.cnki.issn.1674-4152.000142

北京儿童医院2013—2017年住院儿童肺炎链球菌分离株的血清型分析

doi: 10.16766/j.cnki.issn.1674-4152.000142

刘莹¹,
董方²,
史伟¹,
刘钢³,
钱素云⁴,
徐保平⁵,
王青¹,
孟庆红¹,
申昆玲⁵,
姚开虎^1, ,

1. 国家儿童医学中心首都医科大学附属北京儿童医院北京市儿科研究所微生物研究室, 北京 100045;
2. 首都医科大学附属北京儿童医院检验科, 北京 100045;
3. 首都医科大学附属北京儿童医院感染科;
4. 首都医科大学附属北京儿童医院重症医学科;
5. 首都医科大学附属北京儿童医院呼吸科

基金项目:

国家科技支撑计划课题(2013BAI09B11)；国家自然科学基金(81371853)

详细信息

通讯作者:
姚开虎,E-mail:yaokaihu@bch.com.cn

中图分类号: R378.12;R446.5
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- 文章访问数: 301
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- 被引次数: 0
出版历程
- 收稿日期: 2018-01-05
- 网络出版日期: 2022-07-22

Study on serotypes of Streptococcus pneumoniae isolated from hospitalized child in Beijing Children’s Hospital (2013-2017)

Microbiology Laboratory of Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China

摘要

摘要: 目的分析分离自住院患儿的肺炎链球菌的血清型分布,评估肺炎链球菌结合疫苗(PCV)预防感染的价值。方法以2013年3月-2017年12月从北京儿童医院住院的751例患儿临床标本分离出的肺炎链球菌菌株为研究对象,采用荚膜肿胀试验对菌株进行血清分型。结果 5年共收集非重复病例肺炎链球菌751株,其中非侵袭性菌株684株,侵袭性菌株67株。非侵袭性菌株主要分离自呼吸道分泌物(52.3%)和支气管灌洗液(44.4%),侵袭性菌株主要来自血液(50.7%)、胸腔积液(25.4%)和脑脊液(23.9%)。751株菌株中常见血清型为19F(37.0%)、19A(14.2%)、23F(9.5%)、14(7.9%)、6A(7.7%)和6B型(5.2%),共占总血清型的81.5%。PCV10、PCV13和PPV23的覆盖率分别为61.5%、84.3%和82.6%。非侵袭性菌株常见血清型为19F(37.3%)、19A(13.5%)、23F(9.5%)、6A(8.0%)、14(7.0%)和6B(5.6%),PCV10和PCV13的覆盖率分别为60.8%和83.0%。侵袭性菌株常见血清型为19F(34.3%)、19A(22.4%)、14(16.4%)、23F(9.0%)、6A(4.5%)、9V(3.0%)和1型(3.0%),PCV10和PCV13的覆盖率分别为68.7%和95.5%。结论北京儿童医院住院患儿分离的肺炎链球菌以19F、19A、23F、14、6A和6B型常见,PCV13覆盖率超过80%,在侵袭性感染株中更高,推广接种PCV13能够预防绝大多数肺炎链球菌感染。
- 肺炎链球菌 /
- 血清型 /
- 疫苗 /
- 儿童
Abstract: Objective To investigate the situation of serotype distribution of Streptococcus pneumoniae (S. pneumoniae) isolated from Chinese hospitalized children and to evaluate the value of pneumococcal conjugate vaccine in the prevention of S. pneumoniae infection. Methods S. pneumoniae strains isolated from clinical specimens of 751 children hospitalized in Beijing Children's Hospital between March, 2013 and December, 2017 were studied. The serum type was detected by capsule swelling experiment. Results A total of 751 isolates of S. pneumoniae were collected, including 684 non-invasive strains and 67 invasive strains. The non-invasive strains were mainly isolated from respiratory secretions (52.3%) and bronchoalveolar lavage fluid (44.4%). The invasive strains were mainly isolated from blood (50.7%), pleural effusion (25.4%) and cerebrospinal fluid (23.9%). The common serotypes of the 751 strains were 19F (37.0%), 19A (14.2%), 23F (9.5%), 14 (7.9%), 6A (7.7%) and 6B (5.2%), accounting for 81.5%. The coverage rates of PCV10, PCV13 and PPV23 were 61.5%, 84.3% and 82.6%, respectively. The most common serotypes of non-invasive strains were 19F (37.3%), 19A (13.5%), 23F (9.5%), 6A (8.0%), 14 (7.0%) and 6B (5.6%). The coverage rates of PCV10 and PCV13 were 60.8% and 83.0%, respectively. The most common serotypes of invasive strains were 19F (34.3%), 19A (22.4%), 14 (16.4%), 23F (9.0%), 6A (4.5%), 9V (3.0%) and 1 (3.0%). The coverage rates of PCV10 and PCV13 were 68.7% and 95.5%, respectively. Conclusion Serotype 19F, 19A, 23F, 14, 6A and 6B, all of which were included in PCV13, were the common types among the pneumococcal strains isolated from hospitalized children in Beijing Children's Hospital. The coverage rate of PCV13 is more than 80%, and the coverage rate is higher in the invasive infection strains, indicating the promotion of PCV13 can prevent the overwhelming majority of S. pneumoniae infection.
- Streptococcus pneumoniae /
- Serotype /
- Vaccine /
- Children