Abstract: Objective To investigate the effect of New Biological Dressing Type II in the treatment of second degree burn wounds. Methods A total of 36 cases second degree burns in our hospital between November, 2015 and October, 2016 were enrolled into this study and randomly divided into observation group and control group. The observation group was treated with a new biological dressing type Ⅱ, while the control group was treated with silver zinc cream skin and mucous membrane antibacterial agent. The number of dressing, wound healing time, hospitalization time, degree of secondary dressing wound, comfort during changing dressing, comfort to dressing and the wound scar within six months after the discharge were compared between the two groups. Results The number of dressing sessions in the observation group[(4.39±1.14) times] was significantly less than that in the control group[(10.72±3.39) times]; the incidence of fever on day 7 in the observation group was 11.11%, which was significantly lower than that in the control group (50.00%); the wound healing time[(15.67±5.05) days] and hospital stay[(17.22±6.46) days] in the observation group were significantly lower than those in the control group[(21.94±5.47) days and (26.06±10.07) days), respectively]; the degree of secondary dressing wound, comfort during changing dressing and comfort to dressing in the observation group were significant better than those in the control group, the difference was statistically significant (P<0.05); Vancouver Scar Scale scores were 3.44±0.98 in the observation group and 4.94±1.55 in the control group, the difference was statistically significant (P<0.05). Conclusion The new biological dressing type II for second degree burn wounds can reduce the number of dressing, accelerate wound healing, reduce hospital stay, reduce the pain of patients, and reduce the wound scar hyperplasia, worthy of clinical application.