Abstract: Objective The application value of double staining endoscopy in the diagnosis of early digestive tract cancer by grouping comparison. Methods Total 752 cases of gastrointestinal endoscopy patients were selected from May, 2015 to June, 2017, and divided into groups according to different examination methods. The control group (490 cases) received routine endoscopic examination, suspicious lesions were detected by biopsy, and the observation group (262 cases) underwent double staining under endoscopy, two groups of patients with colorectal mucosa, gastric mucosa, esophageal mucosal lesions, early detection of cancer were compared. Data processing with SPSS 19.0 software. Results A total of 57 cases of colorectal lesions were detected in the observation group, the detection rate was 80.28%, 37 cases of colorectal mucosa lesions were detected in the control group, the detection rate was 25.52%, the difference between the two groups was statistically significant (P<0.05); The detection rate of colorectal early cancer was 5.63%, and no early colorectal cancer was found in the control group. The difference between the two groups was statistically significant (P<0.05); A total of 56 cases of gastric mucosal lesions were detected in the observation group, the detection rate was 64.37%, and 24 cases of gastric mucosal lesions in the control group were detected, the detection rate was 14.81%, the difference between the two groups was statistically significant (P<0.05); The detection rate of early gastric cancer was 4.60%, and the early gastric cancer was not detected in the control group. The difference between the two groups was statistically significant (P<0.05); A total of 19 cases of esophageal mucosa lesions were detected in the observation group, the detection rate was 18.27%, and 8 cases of esophageal mucosal lesions in the control group were detected, the detection rate was 4.37%, the difference between the two groups was statistically significant (P<0.05); The detection rate of early esophageal cancer was 3.85%, and no early esophageal cancer was found in the control group. The difference between the two groups was statistically significant (P<0.05). Conclusion Double staining endoscopy can significantly improve the detection rate of early digestive tract cancer. Provide effective guidance for the diagnosis and treatment of disease, and is worthy of promotion.