Abstract: Objective To observe the efficacy of traditional Chinese medicine decoction (Zhengan Xifeng Decoction) in patients with kidney-yin deficiency type premature ovarian insufficiency (POI) and its effects on follicle stimulating hormone, luteinizing hormone and estradiol. Methods From January, 2015 to December, 2016, 64 patients with kidney-yin deficiency type POF were randomly divided into control group and experimental group (32 cases in each group). Patients were treated with hormone replacement therapy (HRT) and Tranquilizing Liver-wind decoction combined with HRT. The symptom scores, hormone (such as FSH, LH and E₂), antral follicles count (AFC) were comparatively analyzed. Results After treatment, the sweating, mood swings, fatigue, headache, skin ants, sexual life, less/amenorrhea syndrome scores and total score in the experimental group were significantly lower than those in the control group (P<0.05). There was no significant difference in menstrual status (such as the number of busts, menstrual cycle, and passage time) after treatment. The total effective rate of the control group and the experiment were 93.75% and 75.00% respectively (P<0.05). After treatment, FSH and LH levels were significantly decreased in both groups, E₂ and AFC were significantly increased, and the improvement of the experimental group was more obvious than the control group (P<0.05). There was no significant difference in the incidence of adverse reactions between the control group and the experimental group (18.75% and 9.38%, P>0.05). Conclusion Compared with simple hormone replacement therapy, combined with Zhengan Xifeng decoction in patients with kidney-yin deficiency type premature ovarian failure has a high clinical efficacy, can effectively regulate the hormone levels, and promote the recovery of ovarian reserve function.