Abstract: Objective To observe the application of ¹⁸F-FDG PET-CT-CIK in the treatment of diffuse large ¹⁸F-FDG cell lymphoma in patients with newly diagnosed diffuse large B cell lymphoma. Methods From the Department of Hematology in our hospital patients with diffuse large B cell lymphoma in 140 cases, all patients were given high CIK cells combined with R-CHOP chemotherapy before chemotherapy and after 3 cycles of chemotherapy, the traditional CT and ¹⁸F-FDG PET-CT scan on patients before treatment, compared with the traditional ¹⁸F-FDG PET-CT CT examination results, and according to the response to chemotherapy were divided into complete response group, partial response group and non-response group three groups, each group of patients before and after treatment were the maximum standard uptake(SUV_max), and the rate of complete remission. Results Before treatment, compared with the traditional CT, the sensitivity of ¹⁸F-FDG PET-CT in the diagnosis of lymph node region and extranodal lesions, the accuracy is significantly higher, the difference was statistically significant (P<0.05). After the treatment, the complete response group, partial response in group SUV_max was significantly lower (P<0.05); the SUV_max value before treatment compared. Complete response group, partial response group, non-response group (P<0.05); compare the value of SUV_max after treatment, complete response group < partial response group < non response group, the difference was statistically significant (P<0.05). Three groups of patients with complete remission rate, complete response group, partial response group, non-response group, the difference was statistically significant (P<0.05). Conclusion ¹⁸F-FDG PET-CT diagnostic sensitivity of diffuse large B cell lymphoma, the accuracy is higher than that of the traditional CT, ¹⁸F-FDG, PET-CT examination in the autologous CIK cells combined with chemotherapy treatment period, can clear to treatment with drug reaction, predictive value of the therapeutic effect and prognosis.