Abstract: Objective To investigate the effect of Shenmai injection with hemoperfusion on myocardial depressant factor(MDF) and cardiac function in septic shock. Methods In this study, 60 patients with septic shock in our Department were randomly divided into 3 groups with 20 cases in each group:the control group, the Shenmai injection group and the Shenmai injection with hemoperfusion group. All the patients received regular therapy. The patients in the Shenmai injection group were treated with Shenmai injection on the basis of the control group. The patients in the Shenmai injection with hemoperfusion group were treated with hemoperfusion(HA330) on the basis of the Shenmai injection group. The levels of APACHEⅡ score, cardiac index(CI), brain natriuretic peptide(BNP), high mobility group protein B1(HMGB-1), leukotriene(LT) and endothelin1(ET-1) before treatment and after seven days of treatment were observed. Results APACHEⅡ score, CI, BNP, HMGB-1, LT and ET-1 among the three groups were not significantly different from each other before the treatment(P>0.05). Compared with those before the treatment, APACHEⅡ score, BNP, HMGB-1, LT and ET-1 in all the three groups decreased after the treatment, CI increased after the treatment, and it was statistically significant(P<0.05). Compared with the control group and the Shenmai injection group, APACHE Ⅱ score, BNP, HMGB-1, LT and ET-1 in the Shenmai injection group were decreased after treatment, and CI was increased, and it was statistically significant(P<0.05). Compared with the Shenmai injection group and the Shenmai injection with hemoperfusion group, APACHE Ⅱ score, BNP, HMGB-1, LT and ET-1 in the Shenmai injection with hemoperfusion group were decreased after the treatment, and CI was increased, and it was statistically significant(P<0.05). Conclusion Shenmai injection with hemoperfusion can reduce the level of myocardial depressant factors, improve the ability of cardiac function and the survival rate of patients in septic shock.