Abstract: Objective To investigate the application and clinical study of three dimensional contrast enhanced ultrasound (CEUS) in the space imaging of gallbladder bed, the purpose is to provide a certain reference value for clinical. Methods One hundred patients with acute cholecystitis underwent laparoscopic cholecystectomy from December, 2015 to December, 2016 were selected from our hospital. According to the random number table method, divided into observation group 50 cases and control group 50 cases. The control group was treated with conventional two-dimensional ultrasound, and the observation group was examined by three-dimensional ultrasound. The amount of intraoperative blood loss, the display of blood vessels in the arterial phase and the effusion of the gallbladder were compared between the two groups. Results The amount of intraoperative bleeding (14.38±3.15) ml less than that of the control group (27.84±5.46) ml (P<0.05). The observation group showed arterial vascular score of 1 was significantly less than the control group, and the 3 was more than the control group (all P<0.05). The gallbladder fossa fluid incidence rate in observation group of (2.00%) was less than that in the control group (20.00%), P<0.05. Conclusion Three dimensional ultrasound technology is a new technique for noninvasive, safe and efficient in gallbladder bed imaging, can be repeated, can reduce the amount of bleeding, reduce gallbladder fossa hydrops, has important diagnostic value.