Abstract: Objective To investigate the effect of empathy mental intervention on the level of hope and cancer related fatigue in the nursing of autologous dendritic cells (DC)/cytokine-induced killer (CIK) cells maintenance in the treatment of advanced pulmonary adenocarcinoma. Methods A total of 100 patients with advanced pulmonary adenocarcinoma who were given the treatment of autologous DC/CIK cells maintenance in Central Hospital of Huzhou from January, 2015 to March, 2017 were selected, and divided into two groups by random number table (n=50):The control group received routine nursing care; the observation group was given empathy mental intervention based on empathy nursing in the control group. The self-rating anxiety scale (SAS) score, self-rating depression scale (SDS) score, piper fatigue scale (PFS) score, Herth hope index (HHI) score and nursing satisfaction were compared between the two groups before and after nursing. Results After nursing, the ASA scores and SDS scores of observation group were significantly lower than those of the control group, with statistical significance (all P<0.05); The PFS score and cognition, feeling, emotion, behavior scores of observation group were significantly lower than those of the control group (all P<0.05); The HHI score and close relationship with others, attitudes towards taking positive action, attitude to the reality and future scores of observation group were significantly higher than those of the control group (all P<0.05); The satisfaction rate of observation group was 96%, which was significantly higher than 82% of control group (P<0.05). Conclusion Empathy mental intervention in the nursing of autologous DC/CIK cells maintenance in the treatment of advanced pulmonary adenocarcinoma can reduce cancer-related fatigue and negative emotion, and obviously improve the patient's level of hope.