Abstract: Objective Oral corticosteroids are commonly used to administrate otitis media with effusion (OME), which majorly caused by mechanical obstruction and infection was routinely treated by tympanic membrane puncture with oral corticosteroids. In this study, triamcinolone acetonide infusion were employed to search for a more effective therapeutics. And the effects were compared with the aformentioned routine therapeutics. Methods A total of 100 cases of OME were randomly divided into experimental group and control group. After auripuncture, the experimental group received intratympanic injection of triamcinolone acetonide, while the control group received oral glucocorticoids treatment. All patients were administrated with Cefaclor Sustained Release Tablets, 0.375 g, b.i.d and ambroxol, 30 mg, t.i.d, for 10 days, and Xylometazoline Hydrochloride Nasal Spray (b.i.d) for 1 week. Pure tone test and acoustic immittance were performed in 2 weeks and 1 month later. All patients were followed up for 3 to 6 months to observe the recurrence rates. Results One month after the treatment, the cure rate in the experimental group and control group was 75.00% (45/60) and 51.85% (28/54); the follow-up showed that the recurrence rate was 6.67% (3/45) in the experimental group and 25.00% (7/28) in the control group. The cure rate was higher in the experimental group than in the control group, the difference in curative effect (cured, effective or not) between the two group was statistically significant (P<0.05). The recurrence rate decreased in the experimental group and the difference was not statistically significant (P>0.05). No complications occurred in both groups. Conclusion Intratympanic triamcinolone acetonide in the treatment of OME is easy to operate, with reliable curative effect and highly safe. It is worthy of clinical promotion.