Abstract: Objective To observe the clinical effect of concentrated growth factors (CGF) combined with Bio-Oss in the treatment of bone defect after jaw cyst surgery, and to provide reference for its popularization and application. Methods A total of 30 cases of medium and large bone defect after jaw cyst surgery in our hospital between July, 2016 and October, 2017 were selected and treated with concentrated growth factors combined with Bio-Oss. The surface of bone defect was covered with double layer concentrated growth factors membrane. The postoperative follow-up was performed at third, sixth, twelfth months after the operation, and the curative effect was evaluated by clinical and imaging examinations. Results All 30 cases were grade I healing after the operation. The imaging examination showed that a large number of new bone formed after 3 months, the bone defect area decreased obviously than preoperative; After 6 months, the new bone density was increased and a large number of bone trabeculae were formed; besides, the new bone was indistinct with the surrounding bone; After 12 months, the density of the bone area was close to the surroundings, the boundary disappeared. The filling material, new bone and surroundings grew well, and the bone defect area was completely repaired, showing good soft tissue and bone tissue effect. Conclusion The concentrated growth factors combined with Bio-Oss can effectively repair the bone defect after jaw cyst surgery and promote soft tissue healing. It is expected to be a new and effective way to apply in clinic.