Abstract: Objective The incidence of prediabetes is increasing year by year in recent years, this paper is to investigate the prevalenc of prediabetes and its related risk factors among the middle-aged and elderly people in Humen area to provide the evidence for prevention and cure strategy of prediabetes. Methods One thousand and fifty volunteers were collected by stratified sampling randomly in Humen from January to December in 2016, who were divided into normal glucose tolerance (NGT) group, pre diabetes (PD) group and diabetes (DM) group according to their blood glucose levels. The demography and medical examination databases of volunteers were gathered by questionnaire, The levels of blood-fasting sugar, triglyeride (TG), low density lipoprotein (LDL), serum uric acid (SUA) and glycosylated hemoglobin 1c (HbA1c) in serum were tested by automatic biochemistry analyzer. The prevalence of prediabetes and diabetes mellitus were analyzed by rate, the risk factors of prediabetes were analyzed Logistic regression. Results One thousand and two people had completely finished the questionnaire, the qualified rate was 95.43%, the prevalencs of prediabetes, diabetes mellitus were and HbA1c step up in prediabetes were 23.95%, 7.88% and 55.00% respectively. The levels of SUA, TG, LDL and HbA1c in patients with PD were higher than that in NGT people (P<0.05). Logistic regression analysis showed that aging, family income, past history of illness, body mass indexes (BMI), waistline, TG, LDL and SUA were the independent risk factors for prediabetes. However, the educational level and physical exercise were the protective factors factor for prediabetes. Conclusion The prediabetes is highly prevalent in the middle-aged and elderly population in Humen. We should make the prevention and cure schema as early as possible according to the prediabetes risk factors and protective factors so as to stop diabetes and it's complications happen.