Abstract: Objective A large number of bacteriological and viral aerosols in oral diagnosis and treatment are easy to cause the surrounding environment and the infection of the patients' wounds. The effective and timely absorption of saliva is an effective method to control aerosol pollution in oral clinic. The purpose of this study was to explore the effect of two kinds of cleaning and disinfection methods on air quality. Methods From July, 2016 to June, 2017, two methods were used to clean the pipe. July-December 2016, the routine cleaning and disinfection method was used in the control group. January-June 2017, the test group was based on the control group, during the second flush, the pipe was cleaned with 3 000 ml 1:200-multienzyme cleaning agent. The air in consulting room and the interface of the siphon was sampled a month. After the cleaning and disinfection, the cleanliness and odour of the suction pipe were observed. Results After cleaning and disinfection, the number of air colonies cultured in the two groups were as follows:(2.667±0.970)CFU/(dish·5 min), (5.222±2.579) CFU/(dish·5 min). The test group was lower than the control group, the difference was statistically significant (t=-3.934,P<0.05). The colony number of the salivary suction interface was cultured as follows:(1.889±1.745)CFU/cm², (4.667±3.125)CFU/cm². The test group was lower than the control group, the difference was statistically significant (t=-3.293, P<0.05). The cleaning degree and smell degree of salivary hose in the test group were better than those in the control group. The difference was statistically significant(χ²=10.395, P<0.05). Conclusion The improved cleaning and disinfection method can effectively reduce the number of bacteria in the room air and sideshow, and effectively increase the cleanliness of the suction hose and reduce the odor of the pipe. The improved clean and disinfectant method is simple and easy to use, which is convenient and reliable.