Abstract: Objective To explore the effect of insulin combined with Danhong injection on foot dorsal hemodynamics, markers of serum inflammation and endothelial function in patients with diabetic foot. Methods From February, 2015 to February, 2016, 108 patients with diabetic foot ulcers were selected in the hospital, according to the order of admission and random control table, the patients were divided into the observation group and the control group, with 54 cases in each group. The control group was treated with insulin to control blood sugar and anti infection; meanwhile, insulin, anisodamine and normal saline were applied to the affected part. The observation group was treated with Danhong on the basis of the control group and intravenous drip of Danhong injection. The course of treatment was 4 week, 1 time a day. The hemodynamics of the dorsalis pedis artery, serum inflammatory markers, endothelial function, ulcer area and side effects were observed in the two groups. Results After treatment, the vascular diameter and blood flow in the observation group were significantly greater than those in the control group (P<0.01), and the peak flow velocity was significantly lower than that in the control group (P<0.01); the levels of TNF-α and IL-6, ET and vWF in the observation group were significantly lower than those in the control group, and the difference between the two groups was statistically significant (P<0.01). The ulcer area in the observation group was significantly lower than that in the control group, and the difference was statistically significant (P<0.01). Neither group had significant adverse reactions. Conclusion The combination of insulin and Danhong injection has some effect on foot dorsal hemodynamics, markers of serum inflammation and endothelial function in patients with diabetic foot, which may be of certain clinical value for the treatment of diabetic foot. In the future work, it should be expanded in the sample size and combined with other indicators to carry out a more in-depth study.