Abstract: Objective To analyze the relationship of positive surgical margin and clinical pathological factors in patients with breast invasive lobular carcinoma (ILC). Methods Clinical data of 192 cases of patients with ILC undergoing breast conserving surgery in our hospital from January, 2010 to December, 2016 were selected, and the relationship of positive surgical margin and clinical pathological factors was analyzed by using single factor and multiple factor analysis method. Results Of the 192 patients with ILC, 47 patients had positive margins (24.48%) after the first operation, and 14 cases still had positive margins after the resection, accounted for 29.79%. Univariate analysis showed that the positive margins of ILC patients at the first operation were significantly correlated with ILC tissue type, mammography and ultrasound prompted multi center/multifocal or structural disorder were closely related with the first positive margin (P<0.05), but there was no significant correlation among age, menopause, tumor size, histological grade, TNM stage, axillary lymphatic metastasis, surgical resection range, ER, PR and HER-2 expression (all P>0.05). The positive margins of ILC patients after two resections were significantly correlated with mammography and ultrasound prompted multi center/multifocal or structural disorder were closely related with the first positive margin (all P<0.05), but there was no significant correlation between age, menopause, tumor size, histological grade, TNM stage, tissue type axillary lymphatic metastasis, surgical resection range, ER, PR and HER-2 expression (all P>0.05). Multivariate Logistic regression analysis showed that mammography and ultrasound indicated multifocal/multifocal or structural disturbances were independent risk factors for positive resection margins of patients with ILC. Conclusion The positive rate of margin resection after breast-conserving surgery in ILC patients is relatively high, and closely related to the presence of structural disorder, multifocal or multifocal presence of mammography or ultrasound. More attentions should be paid to that in clinical.