Abstract: Objective To develop the neonatal eye disease screening information system and explore its significance in neonatal eye disease screening, referral, information feedback and follow-up management. Methods Because the regional neonatal eye disease screening rate was low at present and many problems were faced to the information management of screening data, and the city, county, township three class neonatal eye screening network was relied, so the neonatal eye disease screening information system was developed and increased in child health care information system. According to the technical specifications of children's eye and vision health care, the contents and methods of neonatal eye disease screening were unified and standardized. This system was used to enter the neonatal eye screening results, diagnosis, treatment of neonatal ophthalmopathy, referral, information feedback and follow-up data through the network platform of city maternal and child health care information system. Those screening abnormal newborns were transferred through this system, and the information of children without timely review were displayed on the main interface. At the same time, the system was used to statistical analysis and management of neonatal eye screening in this region. Results The neonatal eye disease screening information system was simplicity of operator. The regional network management of neonatal eye screening was realized and standardized by using data query, statistical reports, network referral, information feedback and management functions, so more neonatal ophthalmopathy be found, diagnosed and treated early. Conclusion The integrated network management function of neonatal eye disease screening-diagnosis-intervention-follow-up-quality control would be met. It is of great significance to establish and apply the neonatal eye screening, because the screening rate and follow-up management would be improved in this region, and each screening unit can realize the construction and sharing of complete screening data resources.