Abstract: Objective To observe the clinical effect of Zishen Liangxue Tiaozhou method in the treatment of endometriosis with infertility patients,and to explore the mechanism of this method to improve the pregnancy rate of patients. Methods According to the r-AFS staging method, 146 patients were divided into the mild group 64 cases (32 cases of the chinese herbal group and the control group) and the moderate to severe group 82 cases (41 cases of the Chinese herbal group and the control group). The mild group:the chinese herbal group were treated with Liangxue Zishen Tiaozhou method from the beginning of the first menstruation for three menstrual cycle, and then observed three menstrual cycle; the control group were observed six menstrual cycles without medication after surgery. The middle to severe group:the Chinese herbal group and the control group were both injected subcutaneously with GnRH-a 3.6 mg on the first day of menstruation, once every four weeks, a total of three needles, and the next treatment were the same as the mild group. Before and after treatment, to compare the improvement of the TCM syndromes, the change of serum levels of VEGF, and the condition of pregnancy within six months after treatment. Results ① The TCM syndrome integral all significantly improved than before in each groups (P<0.01), and between the groups, the Chinese herbal group were both better than the control group (P<0.05). ② The serum of VEGF were all significantly lower than before in each groups (P<0.01), and between the groups, the Chinese herbal group were both better than the control group (P<0.05). ③ The pregnancy rate:the mild group:the chinese herbal group was 46.88%, the control group was 21.88%; the moderate to severe group:the chinese herbal group was 43.90%, and the control group was 21.95%; and between the groups, the Chinese herbal group were both higher than the control group (P<0.05). Conclusion The Liangxue Zishen Tiaozhou method can significantly improve the clinical symptoms of endometriosis with infertility patients, obviously decrease the serum levels of VEGF, and increase the pregnancy rate. The mechanism might be inhibiting excessive expression of VEGF and blocking the formation of neovascularization with ectopic foci.