云南白药对牙龈炎的治疗效果及对口腔破骨细胞功能的影响

刘俊; 和红兵; 任晓斌

doi:10.16766/j.cnki.issn.1674-4152.000587

云南白药对牙龈炎的治疗效果及对口腔破骨细胞功能的影响

doi: 10.16766/j.cnki.issn.1674-4152.000587

昆明医科大学附属口腔医院牙周病科, 云南昆明 650106

基金项目:

国家自然科学基金项目（81660184）

详细信息

通讯作者:
和红兵,E-mail:1320058043@qq.com

中图分类号: R781.41
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出版历程
- 收稿日期: 2018-07-23
- 网络出版日期: 2022-08-04

Curative effect of Yunnan Baiyao on gingivitis and its influence on function of oral osteoclast

Department of Periodontics, the Affiliated Stomatological Hospital of Kunming Medical University, Kunming, Yunnan 650106, China

摘要

摘要: 目的探究云南白药对牙龈炎的治疗效果及对口腔破骨细胞功能的影响。方法选取于2016年6月-2018年6月在昆明医科大学附属口腔医院进行治疗的牙龈炎患者100例,随机分为云南白药组和对照组各50例,云南白药组患者患病部位给予云南白药溶液冲洗,对照组使用盐酸米诺环素软膏进行治疗。收集健康牙槽骨和病变区牙槽骨,进行体外培养和传代。比较患者的牙龈指数(GI)、龈沟出血指数(SBI)、菌斑指数(PLI);采用酶联免疫吸附法检测血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、骨钙蛋白(OC)水平;观察口腔破骨细胞形态;比较2组患者口腔破骨细胞增殖能力;免疫透射比浊法检测Ⅰ型胶原mRNA蛋白表达量;双抗体夹心法检测碱性磷酸酶(ALP)水平。结果治疗后云南白药组患者GI、SBI、PLI值低于对照组(均P < 0.05);治疗后云南白药组患者TNF-α、IL-1β、IL-6、IL-8水平低于对照组(均P < 0.05);在不同时间点,云南白药组口腔破骨细胞增殖率、碱性磷酸酶活性均低于对照组,抗酒石酸酸性磷酸酶活性高于对照组(均P < 0.05);治疗后云南白药组Ⅰ型胶原mRNA蛋白水平、ALP活力、OC水平低于对照组(均P < 0.05)。结论云南白药能够有效抑制牙龈炎患者口腔破骨细胞增殖,促进分化,治疗效果显著。
- 云南白药 /
- 牙龈炎 /
- 口腔破骨细胞
Abstract: Objective To explore the curative effect of Yunnan Baiyao on gingivitis and its influence on the function of oral osteoclast. Methods A total of 100 cases of gingivitis treated in our hospital from June 2016 to June 2018 were randomly divided into the Yunnan Baiyao group and the control group with 50 cases in each group. Yunnan Baiyao group were given the Yunnan Baiyao solution to wash the affected part, and the control group was treated with minocycline hydrochloride ointment. Healthy and lesion area alveolar bone were collected for in vitro culture and subculture. The gingival index (GI), gingival groove haemorrhage index (SBI) and plaque index (PLI) was compared. The serum levels of tumor necrosis factor-alpha (TNFbeta), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8) and osteocalcin (OC) were measured by enzyme-linked immunosorbent assay. The morphology of oral osteoclasts was observed. The proliferation of oral osteoclasts was compared between the two groups. The mRNA expression of Type Ⅰ collagen was detected by immune transmission turbidimetry test. The level of alkaline phosphatase (ALP) was detected by double antibody sandwich method. Results After the treatment, the levels of GI, SBI and PLI in the Yunnan Baiyao group were lower than those of the control group (P < 0.05). After the treatment, the levels of TNF-beta, IL-1β, IL-6 and IL-8 in the Yunnan Baiyao group were lower than those in the control group (P < 0.05). At different time points, the proliferation rate and alkaline phosphatase activity of oral osteoclasts in the Yunnan Baiyao group were low compared with the control group, and the activity of tartaric acid acidic phosphatase was higher than that of the control group (P < 0.05). The mRNA level of type Ⅰ collagen protein, ALP activity and OC level in the Yunnan Baiyao group is lower than those in the control group after the treatment (P < 0.05). Conclusion Yunnan Baiyao can effectively inhibit the proliferation of osteoclasts in the oral cavity of gingivitis patients, promote differentiation, with a remarkable curative effect.
- Yunnan Baiyao /
- Gingivitis /
- Oral osteoclast