Abstract: Objective To investigate the infection and epidemiological characteristics of rotavirus (RV) and adenovirus (Adv) in babies with diarrhea in Lishui. Methods The 1 133 cases of feces form children with diarrhea were collected from January to December 2016 in Zhejiang University Lishui hospital, and immunochromatography technique was used to diagnose RV and Adv antigens in feces of children, and the results were statistically analyzed. Results Among the 1 133 cases of diarrhea, 222 cases of rotavirus were detected, the infection rate was 19.59%. The adenovirus was 23 cases, the infection rate was 2.03%, and there were 17 cases coinfection of rotavirus and adenovirus, the infection rate was 1.5%. In male patients, the positive rate of rotavirus infection was 20.18% (134/664), the adenovirus was 2.79% (18/664), and double infection was 1.96% (13/664). In female patients, the positive rate of RV, adenovirus and double infection were18.76% (88/469), 1.07% (5/469) and 0.85% (4/469), respectively. There was no significant difference in the positive rate of gender (P > 0.05). The positive rate of rotavirus decreased with the age increase (P < 0.01). The highest positive rate of rotavirus in children was 6 to 12 months. The highest incidence of rotavirus infection in this region from November to next year February, a total of 144 cases were positive, accounting for 64.86% (χ²=57.745, P < 0.001); high incidence of adenovirus infection in May to June, 14 cases were positive, accounting for 60.86% (χ²=113.900, P < 0.001).The highest incidence of rotavirus and adenovirus double infection was from December to January of the following year, of which 12 cases were positive, accounting for 70.58% (χ²=24.852, P < 0.001). Conclusion Rotaviruses are the main pathogens for children with diarrhea in the region, and the onset of virus infection has obvious seasonal change. In particular, paying attention to infants and young children with diarrhea infection with rotavirus and adenovirus, select a fast and accurate diagnostic method for clinical diagnosis and treatment are necessary.