Abstract: Objective Prostate specific antigen (PSA) is one of the important methods of prostate cancer screening, but its specificity is not high. This study aims to explore the association between serum PSA level and Body Mass Index (BMI), age and International Prostate Symptom Score (IPSS) in non-prostate cancer individuals. Methods Serum PSA level, demographic information (age, education, marital status, etc.) and various clinical parameters (height, weight, IPSS, etc.) were collected through questionnaire investigation and laboratory testing from 1 575 non-prostate cancer male in the community, using cluster random sampling. Student's t test. Analysis of Variance, Chi-square test, and unconditional multivariate logistic regression was conducted to analyze the potential influence factors of the serum PSA level. Results The average serum PSA level was 0.60 (0.32-1.12)ng/mL, of which 55 samples were greater than or equal to 4.00 ng/mL, accounting for 3.5% of the total number. The serum PSA was associated with age and IPSS scores and had no correlation with other clinical parameters. Conclusion The increase of age and IPSS score in non-prostatic cancer individuals are the risk factors for the increase of serum PSA level. The diagnosis of prostate cancer acquires the combination of the serum PSA measurement and other methods in order to reduce the false positive rate.