Abstract: Objective To study the clinical effect of comfort care in chronic nonspecific ulcerative colitis. Methods A total of 130 cases of patients with chronic nonspecific ulcerative colitis were selected from April 2016 to April 2018 treated in our hospital. According to the different methods of nursing care, they were divided into routine nursing group and comfortable nursing group, with routine nursing group receiving routine nursing intervention and comfortable nursing group receiving comfortable nursing intervention in six aspects including medical care, psychological care, music, nursing, enema, diarrhea nursing and diet nursing. Double antibody clamp method was used to detect the Tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) was used to measure patients' life quality, nursing effect and post-nursing satisfaction. Results After the nursing intervention, the levels of TNF-α and IL-6 in the comfort nursing group were significantly lower than those in the conventional nursing group, with statistically significant differences (all P<0.05). The quality of life of patients in the post-nursing comfort care group was better than that in the conventional care group, and the difference was statistically significant (all P<0.05). The total nursing efficiency of comfort nursing group was higher than that of conventional nursing group, and the difference was statistically significant (all P<0.05). The overall satisfaction rate of patients in the comfort nursing group was higher than that in the conventional nursing group, and the difference was statistically significant (P<0.05). Conclusion Comfort care can effectively improve the level of inflammatory factors in patients with chronic nonspecific ulcerative colitis, promote the physical recovery of patients and make patients more satisfied.