节律基因NPAS2在急性髓系白血病中的表达及对细胞生长的影响

牟佼; 杨西亮; 任婧婧; 宋艳萍

doi:10.16766/j.cnki.issn.1674-4152.000690

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节律基因NPAS2在急性髓系白血病中的表达及对细胞生长的影响

doi: 10.16766/j.cnki.issn.1674-4152.000690

1. 西安市中心医院血液病研究所, 陕西西安 710003;
2. 空军94259部队卫生队, 山东蓬莱 265600

基金项目:

陕西省自然科学基础研究计划资助项目(2015JM8479，2017JM8025)

详细信息

通讯作者:
宋艳萍,E-mail:xjtusyp@163.com

中图分类号: R733.71
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- HTML全文浏览量: 26
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- 被引次数: 0
出版历程
- 收稿日期: 2018-08-28

Expression of NPAS2 in acute myeloid leukemia and its effect on cell growth

Department of Hematology, Xi'an Central Hospital, Xi'an, Shaanxi 710003, China

摘要

摘要: 目的探讨神经元PAS结构域蛋白2(neuronal PAS domain protein 2,NPAS2)在急性髓系白血病(acute myeloid leukemia,AML)患者和细胞系中的表达变化,及其对AML细胞生长的作用。方法收集陕西省西安市中心医院血液病研究所2013-2017年收治的初诊急性髓系白血病患者46例,实时定量PCR方法检测NPAS2在AML患者及正常对照组骨髓单个核细胞中的表达,Western blot方法检测AML细胞系及对照组中NPAS2的表达;统计学方法分析NPAS2表达高低对AML患者预后的影响;RNAi方法干扰AML细胞内NPAS2表达,利用CCK-8法检测NPAS2对细胞生长的作用。结果 NPAS2在AML患者及细胞系中的表达较对照组升高(P<0.05);在M1、M2、M3、M4、M5患者中NPAS2表达较对照组升高(P<0.05),在M6和M7患者中其表达较对照组差异无统计学意义(P>0.05);高表达NPAS2的AML患者其总体生存期较低表达患者短(P<0.05);AML细胞中干涉NPAS2后,与对照组比,干涉组细胞生长明显减慢(P<0.05)。结论 NPAS2在AML患者中表达上调并与AML患者预后负相关,同时NPAS2促进AML细胞生长。
- 神经元PAS结构域蛋白2 /
- 表达 /
- 预后 /
- 生长 /
- 急性髓系白血病
Abstract: Objective To explore the expression of NPAS2 in AML patients and cell lines, and to analyze the effect of NPAS2 on the growth of AML cells. Methods The mRNA expression of NPAS2 in 46 AML patients was analyzed from 2013 to 2017 by real-time PCR. The protein expression of NPAS2 in AML cell lines was analyzed by western blot. The relationship between the expression of NPAS2 and prognosis of AML patients was analyzed by statistical method. The effects of NPAS2 on the growth of AML cells were detected by using CCK-8 after using RNAi to interfere the expression of NPAS2. Results The expression of NPAS2 in AML patients and cell lines was higher than that in control group (P<0.05). Compared with control group, the expression level of NPAS2 in the AML-M1, M2, M3, M4 and M5 patients was higher (P<0.05), and the expression level in the AML-M6 and M7 patients was no different (P>0.05). AML patients with high expression of NPAS2 had a shorter overall survival period (P<0.05). Compared with the control group, the cell growth of the RNAi-NPAS2 group was significantly decreased in AML cells (P<0.05). Conclusion The expression NPAS2 was raised in AML patients and negatively correlated with the prognosis of AML patients, while NPAS2 promoted AML cell growth.
- NPAS2 /
- Expression /
- Prognosis /
- Growth /
- Acute myeloid leukemia