牛膝多糖对老年骨质疏松大鼠模型骨代谢及生物力学特征的影响

郎小琴; 高越; 周叶; 陈美红; 徐楚韵

doi:10.16766/j.cnki.issn.1674-4152.000730

牛膝多糖对老年骨质疏松大鼠模型骨代谢及生物力学特征的影响

doi: 10.16766/j.cnki.issn.1674-4152.000730

1. 浙江大学医学院附属杭州市第一人民医院综合病区, 浙江杭州 310006;
2. 浙江中医药大学基础医学院, 浙江杭州 310053

基金项目:

浙江省中医药科技计划项目(2014ZB079)

详细信息

通讯作者:
高越,E-mail:qoptksf@sina.com

中图分类号: R681;R446.62
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出版历程
- 收稿日期: 2018-10-18

Influence of achyranthan on bone metabolism and biomechanical characteristics of elderly rats model of osteoporosis

Comprehensive Ward of the First People's Hospital of Hangzhou, Hangzhou, Zhejiang 310006, China

摘要

摘要: 目的探讨牛膝多糖对老年骨质疏松大鼠模型骨代谢及生物力学特征的影响。方法将120只10月龄雌性SD大鼠随机分为6组，除对照组和假手术组，均以双侧卵巢摘除术制备骨质疏松模型。术后饲养3个月，低、中、高剂量组以100、200、400 mg/(kg·d)的牛膝多糖灌胃。采用ELISA实验测腹腔静脉血骨钙素(OC)、骨特异性碱性磷酸酶(BAP)、抗酒石酸酸性磷酸酶(TPACP5b)、Ⅰ型胶原交联N-末端肽(NTX)与Ⅰ型胶原交联C-末端肽(CTX)水平；WB实验测定股骨与胫骨骨髓组织核因子κB受体活化因子(RANK)、RANK配体(RANK ligand，RANKL)及骨保护素(OPG)表达；液压伺服材料试验机测定椎体及股骨的最大应力和断裂吸收能量。结果与对照组、假手术组相比，模型组大鼠的血清OC和BAP含量，骨髓组织OPG和RANK表达降低，血清TPACP5b、NTX、CTX含量，骨髓组织RANKL表达升高；与模型组相比，低剂量组、中剂量组、高剂量组大鼠血清OC和BAP含量，骨髓组织OPG和RANK表达依次升高，血清TPACP5b、NTX、CTX含量，骨髓组织RANKL表达依次降低，组间差异有统计学意义(P＜0.05)；与对照组、假手术组相比，模型组大鼠的椎体和双侧股骨的最大应力及断裂吸收能量明显降低；与模型组相比，低剂量组、中剂量组、高剂量组大鼠的最大应力及断裂吸收能量依次升高；组间差异有统计学意义(P＜0.05)。结论牛膝多糖能够改善老年骨质疏松大鼠的骨代谢，提高骨密度和骨抗压能力，在抗骨质疏松领域具有潜在的应用价值。
- 牛膝多糖 /
- 骨质疏松 /
- 骨代谢 /
- 生物力学
Abstract: Objective To explore the influence of achyranthan on bone metabolism and biomechanical characteristics of elderly osteoporosis model rats. Methods Totally 120 female SD rats were divided into 6 groups. In addition to the rats in the control group (Control) and the sham operation group (SO), the others were prepared for osteoporosis modes by surgical castration. After the surgery, the rats in intervention 1, 2, 3 were given intragastric administration with achyranthan of 100, 200, 400 mg/(kg·d), for 3 months. The levels of osteocalcin (OC), bone specific alkaline phosphatase (BAP), tartrate-resistant acid phosphatase (TPACP5b), Ⅰ type collagen cross-linking N-terminal peptide (NTX) and Ⅰ type collagen cross-linking C- terminal peptide (CTX) of abdominal venous blood of rats were detected by ELISA kits. The receptor activator of NF-kappa b (RANK), RANK ligand (RANKL) and osteoprotegerin (OPG) expression of myelosis of rats were detected by WB. Maximum stress and fracture absorb energy of centrum and thighbone of rats was detected by using servo-hydraulic materials testing machines. Results Compared with the Control/SO group, the serum OC and BAP level, and the medullispinal OPG and RANK expression of the model group decreased, and the serum TPACP5b, NTX and CTX level, and the medullispinal RANKL expression of the model group increased. Compared with model group, the serum OC and BAP level, and the medullispinal OPG and RANK expression of intervention 1, 2, 3 group decreased successively, and the serum TPACP5b, NTX and CTX level, and the medullispinal RANKL expression of intervention 1, 2, 3 group increased successively, the difference among the different groups was statistically significant (P＜0.05). Compared with the control/SO group, the maximum stress and fracture absorb energy of the model group decreased. Compared with model group, the maximum stress and fracture absorb energy of the intervention 1, 2, 3 group increased successively, the difference among the groups was statistically significant (P＜0.05). Conclusion Achyranthan can improve bone metabolism, increase bone density and bone compressive ability of elderly osteoporosis rats, and has potential application value in the field of anti-osteoporosis.
- Achyranthan /
- Osteoporosis /
- Bone metabolism /
- Biomechanics