Abstract: Objective To investigate the effects of humanistic care concept combined with respiratory function exercise on pulmonary function, negative emotion and quality of life in patients with lung adenocarcinoma. Methods Eighty-six cases of advanced lung adenocarcinoma patients in our hospital from January 2014 to June 2016 were selected as the research object and were randomly divided into control group and observation group; the control group were given respiratory function exercise, the observation group were given humanistic care concept combined with respiratory function exercise. The lung function of the two groups was evaluated by FEV1 and FEV1/FVC. The emotional state of the two groups was evaluated by SAS and SDS. The quality of life of the two groups was evaluated by 36-SF scale. The lung function, negative emotion, quality of life and treatment compliance were compared between the two groups. Results After 1 month of treatment, the FEV1 (1.66±0.22) in the observation group was significantly higher than that in the control group (1.43±0.24), the FEV1/FVC in the observation group (67.92±10.06) was significantly higher than that in the control group (58.19±9.35). The difference was significant (both P<0.05). The SAS and SDS in the observation group (38.75±2.68, 35.47±3.04) were significantly lower than those in the control group (47.67±4.29, 47.35±4.28), all P<0.05. The quality of life of the observation group was significantly higher than that of the control group. The treatment compliance of observation group (90.70%) was significantly better than the control group (62.80%), P<0.05. Conclusion Humanistic care concept combined with respiratory function exercise can improve the lung function and reduce the negative emotions of pemetrexed combined with cisplatin in patients with lung adenocarcinoma, and improve their quality of life and treatment compliance.