Abstract: Objective To investigate the clinical efficacy of Zhulian Zhenfa acupuncture on polycystic ovary syndrome (PCOS) in obesity women and its influence on life quality of patients. Methods Sixty obesity women with PCOS in our hospital from January 2017 to June 2018 were selected as subject and divided into control group and observation groups according random number table, with 30 cases in each group. The control group was given ordinary acupuncture, while the observation group was given Zhulian Zhenfa acupuncture. The BMI, SDS score, SAS score, life quality (SF-36) and the levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E₂), and testosterone (T) of two groups were compared before and after treatment to investigate effect of Zhulian Zhenfa acupuncture on PCOS. Results After the treatment, the BMI of both groups were significantly decreased (P<0.05), and the observation group was better than the control group (P<0.05). There was no significant difference in SDS and SAS scores in the control group (P＞0.05), however, there was significant difference in the observation group when compared with pretreatment values (P<0.05). After the treatment, the observation group was better than the control group in improving the quality of life (P<0.05). After the treatment, the levels of LH, E₂ and T in the two groups were significantly dcreased (P<0.05), and the observation group was better than the control group (P<0.05). Conclusion Zhulian Zhenfa acupuncture on PCOS in obesity women can significantly improve the state of depression, anxiety and quality of life of patients. The improvement of LH, E₂, T and other sex hormones levels may be the potential mechanism in patients undergoing Zhulian Zhenfa acupuncture.