Abstract: Objective To explore the effect of continuous nursing on the quality of life of patients with human epidermal growth factor receptor 2 (HER2) positive gastric cancer after targeted therapy. Methods A total of 140 patients with HER2-positive gastric cancer who were treated with trastuzumab in our hospital from January 2016 to January 2017 were selected. All patients were randomly divided into continuous nursing group and conventional nursing group, 70 cases in each group. Conventional nursing group used routine nursing measures for nursing, while continuing nursing group used continuing nursing on the basis of routine nursing. The quality of life, depression, anxiety and self-perceived burden of the two groups before and after nursing were evaluated, and the hospitalization time and patients' satisfaction were compared between the two groups. Results After nursing, the scores of quality of life in the two groups were higher than those before nursing, and those in the continuing nursing group were higher than those in the conventional nursing group (all P<0.05); after nursing, the scores of HAMD and HAMA in the two groups were lower than those before nursing, and the scores of HAMD and HAMA in the continuing nursing group after nursing were lower than those in the conventional nursing group (all P<0.05); after nursing, the scores of SPB in the two groups were lower than those in the conventional nursing group and the SPB scores of continuing nursing group were lower than those of routine nursing group (all P<0.05). The length of stay in continuing nursing group was lower than that of routine nursing group (P<0.05), and the total satisfaction of nursing patients in continuing nursing group was higher than that of routine nursing group (P<0.05). Conclusion Continuous nursing intervention after targeted therapy for HER2-positive gastric cancer patients can effectively improve the quality of life of patients, reduce the burden of self-perception, and make patients more satisfied.