Abstract: Objective To observe the inhibitory effect of avasimibe on the growth, metastasis invasion and the effect of ACAT-1 expression on human ovarian cancer SK-OV-3 cell line, thus reflecting the effect of avasimibe on the anti-tumor effect of human ovarian cancer SK-OV-3 cell line. Methods Avasimibe (10 μM) was applied to human ovarian cancer SK-OV-3 cell line to establish an experimental model of SK-OV-3 cell line (ACAT-1 group); avasimibe cloth was screened by CCK8 experiment (10 μM) which was applied to SKOV3 ovarian cancer cell line for the best time (72 h), i.e., ACAT-1-72 group; the control group (i.e., parental ovarian cancer SK-OV-3 cell line) and ACAT-1 were determined by scratch test. The proliferative capacity and the ability of invasion and metastasis in 72 groups were the same; the expression of ACAT-1 in the control group and ACAT-1-72 group was determined by Western blot; the control group and ACAT-1-72 group were detected by immunofluorescence assay for the difference in expression of ACAT-1. Results Compared with the control group, the proliferation ability of tumor cells in the experimental group (ACAT-1 group) was weakened in a time-dependent manner (P<0.05), and the proliferative ability of the ACAT-1-72 group in the experimental group was the most obvious. As the best experimental group for the subsequent three sets of experiments; compared with the control group, the experimental group (ACAT-1-72 group) had weaker invasion and migration ability of tumor cells in the same period of time (P<0.05); compared with the control group, the expression of ACAT-1 protein in the experimental group (ACAT-1-72 group) was decreased (P<0.05). Conclusion Avasimibe can effectively promote anti-tumor effect on ovarian cancer SK-OV-3 cell lines, and its main mechanism is related to inhibiting the expression of ACAT-1 protein.