Abstract: Objective To explore the application effects of applied anatomy in the teaching of ENT surgery. Methods In 2017, we selected 10 graduate students from the 2015 Department of Otorhinolaryngology Head and Neck Surgery of Anhui Medical University, and ordered 10 students according to the ranking of the test results, the singular was the control group and the double number was the experimental group, with 5 people in each group. On the basis of traditional teaching, the experimental group physicians additionally offered courses such as viewing surgery, anatomical video and hands-anatomy to strengthen the study of applied anatomy. The two groups of students in the control group used experimental teaching to complete a nasal endoscopic sinus opening operation. Test the teaching effect and conduct a questionnaire survey to understand the students' experience of the two teaching methods. Results The results of the surgical examination showed that the average scores of the experimental group and the control group were 82.52 points and 61.02 points. There was a significant difference between the two groups (P<0.05). The scores of the surgical evaluation of the experimental group were significantly higher than those of the control group. The independent completion degree of the operation, the treatment of the sudden situation, the protection of the surrounding tissue, the amount of bleeding, and the operation time were better than the control group. The average teaching evaluation scores of the experimental group and the control group were 25 points and 24 points, respectively. There was no significant difference in the evaluation scores of the two teaching modes (P>0.05), and 90% (9/10) of the students believed that the new teaching methods would help beginners improve their learning satisfaction, learning interest, understanding and learning efficiency of teaching content. Conclusion The course of viewing anatomical video and autopsy anatomy in the teaching of otolaryngology head and neck surgery is a better teaching method and can be further promoted and applied.