Abstract: Objective To investigate the role and mechanism of lncRNA plasmacytoma variant translocation 1 (PVT1) in the proliferation and migration of non-small cell lung cancer (NSCLC) cells by up-regulating or down-regulating the expression of PVT1, and then analyze the target gene of PVT1 by using RNA-Seq for its action mechanism. Methods ① The relationship between the expression of PVT1 in NSCLC cells and the survival time of NSCLC patients through studying TCGA database (The Cancer Genome Atlas); ② Using Ki67 immunofluorescence assay and cell colony formation assay to investigate the effect of up-regulation or down-regulation of PVT1 on the proliferation and migration of NSCLC cells; ③ Using RNA-seq technology to explore the downstream target genes of PVT1, and then use siRNA to silence the gene to explore the ability of this gene to proliferate and invade NSCLC cells. Results ① The high level expression of PVT1 was significantly associated with the decrease of survival time of lung cancer patients (P<0.001). ②The high level expression of PVT1 significantly promoted the increase of tumor volume in lung cancer patients, and was closely related to tumor stage and lymph node metastasis (all P<0.05); ③ The overexpression of PVT1 promoted the proliferation and migration of NSCLC cells; ④Down-regulation of PVT1 by Si-RNA inhibited the proliferation and migration of NSCLC cells; ⑤Down-regulation of PVT1 significantly increased the expression level of LATS2. Conclusion PVT1 can promote the proliferation and migration of NSCLC cells by apparently regulating LATS2.