Abstract: Objective To investigate the molecular basis of Qiangguyin(QGY) and its predictive targets, to clarify the multi-targets characteristics of QGY in the treatment of Postmenopausal osteoporosis. Methods First, the method of system biology was used to determine the known compounds corresponding to the herbs by the Chinese medicine comprehensive database (TCMID). The name of the compound was determined by NCBI data into the unique ID number. And then we put the data of the unique ID number into the molecular mechanism of the Chinese medicine molecular mechanism bioinformatics analysis tool (BATMAN-TCM) to predict the drug-related gene / protein targets, and then use the bioinformatics method to predict the Gene ontology (GO) , signal pathway and protein - protein interaction network of the targets. Results In this study, a total of 435 compounds were included from 12 herbs, and 2 487 targets were retrieved，which were further enriched in 138 GO terms, such as Nucleic Acid Binding Transcription Factor Activity, Signal Transducer Activity, Oxidoreductase Activity (all P<0.05). A total of 211 signal pathways was significantly enriched (all P<0.05). Conclusion The herbs, compounds and targets related to compounds of QGY are effective research carriers for QGY. The prevention and treatment of osteoporosis with QGY is based on multiple target strategies. QGY by coordinating the body's metabolic balance is preventing and treating osteoporosis for the cause of the disease.