Abstract: Objective To explore the incidence of functional constipation in infants, factors during pregnancy and the correlation between them. Methods From January 2016 to June 2016, 263 newborns born in our hospital were selected and were followed up for 2 years, they were divided into constipation group and non-constipation group according to whether functional constipation occurred. The general data of two groups when constipation occurred were compared, the maternal factors during pregnancy of two groups were also compared, logistic regression was used to analyze the independent risk factors of functional constipation. Results Fifty-one cases (19.39%) of functional constipation occurred in this study. The proportion of women in the constipation group was significantly higher than non-constipation group (56.86% vs. 38.35%; χ²=5.751, P=0.016), there were significant differences in maternal blood pressure, blood glucose, BMI before pregnancy and probiotics supplementation during pregnancy between the two groups (P<0.05). Multivariate logistic regression analysis showed prenatal blood pressure, prenatal blood glucose, prenatal BMI were risk factor for functional constipation(P<0.05), and prenatal probiotics supplementation was protective factors for functional constipation (P<0.05), multivariate logistic regression analysis showed that maternal obesity was independent risk factor for functional constipation(OR=1.405, P=0.019), probiotics supplementation was independent protective factors for functional constipation (OR=0.325, P=0.025). Conclusion Infant functional constipation has a certain correlation with maternal factors during pregnancy. Controlling pregnancy weight and supplementing probiotics can effectively reduce the occurrence of constipation.