Abstract: Objective To explore the nursing value of standardized nursing for patients with low response and no response to hepatitis b vaccine after reseeding. Methods A total of 160 middle-aged and elderly people who received multiple vaccination of hepatitis B vaccine from January 2017 to January 2018 in Shaoxing Municipal Hospital were selected. All the subjects were randomly divided into two groups: routine nursing group and standardized nursing group, with 80 cases in each group. The recombinant hepatitis B surface antigen vaccine (yeast) was used to inoculate the deltoid muscle of upper arm of two groups of subjects. The subjects in the routine nursing group were given routine nursing, and the subjects in the standardized nursing group were given standardized nursing. Immunotransmission turbidimetry was used to detect hepatitis B virus markers. A special questionnaire was used to investigate the satisfaction of family members of all the subjects. The incidence and treatment of adverse reactions after vaccination were counted and compared between the two groups. Results After two groups of subjects injected with hepatitis B vaccine and received nursing, the total satisfaction of family members in the standardized nursing group was significantly higher than that in the conventional nursing group, with statistical significance (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups after injection of hepatitis B vaccine (P>0.05). After nursing, all the patients in the standardized nursing group who had adverse reactions were treated in time, which was significantly higher than 36.36% in the conventional nursing group (P<0.05). Conclusion The standardized nursing intervention can timely deal with the adverse reactions after vaccination and make the family members more satisfied.