促红细胞生成素衍生肽对急性心肌梗死大鼠心脏的保护作用

王世祥; 王斌; 郭国锋; 许卫; 陈永权; 陈晞明

doi:10.16766/j.cnki.issn.1674-4152.000873

促红细胞生成素衍生肽对急性心肌梗死大鼠心脏的保护作用

doi: 10.16766/j.cnki.issn.1674-4152.000873

1. 广州医科大学附属第三医院心内科, 广东广州 510000;
2. 菏泽市中医医院放射科山东菏泽 274000;
3. 广州市花都区人民医院心内科, 广东广州 510000

基金项目:

广东省科技厅基金项目(201311079)；广州市医药卫生科技项目(20161A011079)

详细信息

通讯作者:
王世祥,E-mail:630311325@qq.com

中图分类号: R542.22
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出版历程
- 收稿日期: 2018-11-21

Protective effect of erythropoietin derived peptide on heart in rats with acute myocardial infarction

Department of Cardiology, the Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510000, China

摘要

摘要: 目的探讨促红细胞生成素衍生肽(helix B surface peptide，HBSP)对急性心肌梗死(acute myocardial infarction，AMI)大鼠心脏的保护作用。方法将30只8周龄SD雄性大鼠随机分3组：假手术组(sham组)、AMI组和HBSP组，每组10只。采用结扎大鼠冠状动脉前降支术以构建AMI大鼠模型，治疗组为术后予腹腔注射HBSP(90 μg/kg)；假手术组和AMI组给予等量生理盐水腹腔注射。术后24 h心脏彩超检测心功能，处理各组大鼠，以蛋白免疫印迹法检测心肌组织信号传导与转录激活因子3(signal transduction and transcription activator 3，STAT3)、B淋巴细胞瘤-2基因(B-cell lymphoma-2，Bcl-2)、Bax蛋白(BCL2 associated X protein)表达，原位缺口末端标记法(TUNEL法)检测心肌细胞凋亡情况。结果 HBSP治疗组与AMI组比较，大鼠心肌梗死面积及心功能明显改善。AMI组与假手术组比较，STAT3、Bcl-2表达明显下降而Bax表达显著上升(P<0.01)，HBSP组STAT3、Bcl-2表达较AMI组显著升高，而Bax表达显著减少(P<0.01)。与假手术组比较，AMI组心肌细胞凋亡率明显增加(P<0.01)，而HBSP组心肌细胞凋亡率明显降低(P<0.01)。AMI模型组心功能指标显著低于假手术组(P<0.01)，而HBSP治疗组心功能指标明显高于AMI模型组(P<0.01)。结论 HBSP可使STAT3、Bcl-2表达增加抑制急性心肌梗死大鼠心肌细胞凋亡，从而保护心肌组织，改善大鼠心功能。
- 促红细胞生成素衍生肽 /
- 急性心肌梗死 /
- 细胞凋亡
Abstract: Objective To investigate the protective effect of helix B surface peptide (HBSP) on the heart of rats with acute myocardial infarction (AMI). Methods Thirty 8-week-old male SD rats were randomly divided into 3 groups: sham operation group (sham group), AMI group and HBSP group. The AMI rat model was established by ligating the anterior descending coronary artery. The treatment group was intraperitoneally injected with HBSP (90 μg/kg), the sham operation group and AMI group were intraperitoneally injected with the same amount of normal saline. Cardiac function was detected by color Doppler echocardiography 24 hours after operation. The myocardial signal transduction and transcription activator 3 (STAT3), B lymphocytoma 2 gene (Bcl-2) were detected by Western blotting in each group of rats. Apoptosis was detected by expression of Bcl-2, Bax protein (BCL2 associated X protein) and detection of myocardial fineness by in situ Nick end labeling (TUNEL). Results Compared with AMI group, myocardial infarction size and cardiac function of rats in HBSP group were significantly improved. STAT3, Bcl-2 expression in AMI group was significantly lower than that in sham operation group (P<0.01). In HBSP group, STAT3 was significantly higher than that in sham operation group (P<0.01). In HBSP group, the expression of Bcl-2 was significantly higher than that of AMI, while the expression of Bax was significantly decreased (P<0.01). Compared with the sham operation group, the cardiomyocyte apoptosis rate in the AMI group was significantly increased, while that in the HBSP group was significantly lower than that in the sham operation group (P<0.01). AMI model group was significantly lower than that in the sham operation group. The cardiac function in HBSP group was significantly higher than that in AMI model group (P<0.01). Conclusion HBSP can increase the expression of STAT3, Bcl-2 and inhibit the apoptosis of myocardial cells in rats with acute myocardial infarction, thereby protecting myocardial tissue and improving cardiac function in rats.
- Helix B surface peptide /
- Acute myocardial infarction /
- Apoptosis