Abstract: Objective To evaluate the clinical effect of subgingival ultrasonic scaling and subgingival glycine powder air polishing in the treatment of class Ⅱ furcation involvement. Methods Sixty-four class Ⅱ furcation involvement molars from thirty-two severe chronic periodontitis patients in Stomatological Hospital of Anhui Medical University from May 2017 to May 2018 were selected. Everyone had two class Ⅱ furcation involvement molars at least. One week after the supragingival scaling, the clinical index were taken as the baseline data. The principle of oral control design was adopted. One side of the same patient's teeth was randomly designed as experimental group, subgingival scaling and subgingival glycine powder air polishing was performed, the other side as control group, subgingival scaling was performed. After 4 weeks and 12 weeks, the periodontal clinical indicators, inflammatory factor from gingival crevicular fluid and BANA test were reviewed to evaluate the clinical effects. Results The differences between the two groups in baseline were not significant (all P>0.05). The data of 4 weeks and 12 weeks after the treatment were analyzed using repeat measurement test, except CAL, the other observed periodontal clinical index were all significantly improved after the treatment (all P<0.05). The level of tumor necrosis factor-α and interleukin-6 were all lower as compared with those at baseline. BANA results showed that the overall quantity of pathogens decreased in both groups after 4 weeks of treatment (all P<0.05). Twelve week later, the overall quantity of pathogens in the experimental group was still lower than that in the control group, and the difference was significant (P<0.01). Conclusion During 12 weeks of follow-up, subgingival ultrasonic scaling combine subgingival glycine powder air polishing can effectively improve the clinical symptoms and inflammatory response of class Ⅱ furcation involvement, which can maintain the long-term therapeutic effect when compared with subgingival scaling only.