阻滞Kv1.3通道抑制CD4<sup>+</sup>CD28<sup>null</sup> T细胞活性缓解糖尿病微血管损伤的实验研究

余筱燕; 汤珂珂; 吕迪

doi:10.16766/j.cnki.issn.1674-4152.000937

阻滞Kv1.3通道抑制CD4⁺CD28^null T细胞活性缓解糖尿病微血管损伤的实验研究

doi: 10.16766/j.cnki.issn.1674-4152.000937

宁波大学医学院附属医院内分泌科, 浙江宁波 315000

基金项目:

浙江省宁波市医学科技计划项目(2017A18)

详细信息

通讯作者:
余筱燕,E-mail:yoyoyong0123@163.com

中图分类号: R587.2
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出版历程
- 收稿日期: 2018-10-16

Inhibit the activity of CD4⁺CD28^null T cells to alleviate diabetic microvascular damage by blocking Kv1.3 channel

Department of Endocrinology, the Affiliated Hospital of Medical School of Ningbo University, Ningbo, Zhejiang 315000, China

摘要

摘要: 目的探讨通过阻滞Kv1.3通道抑制CD4⁺CD28^null T细胞活性缓解糖尿病微血管损伤的可行性。方法选取宁波大学医学院附属医院2016年1月—2017年12月收治的单纯糖尿病患者(DM组)、糖尿病视网膜病变患者(DR组)、糖尿病肾病患者(DN组)和糖尿病视网膜病变合并肾病患者(DR+DN组)各20例，另以20例健康志愿者为对照组，检测并提取CD4⁺CD28^null T细胞备用。96孔板培养人血管内皮细胞，分为5组：对照组、CD28^null T干预组、Kv1.3阻滞1组、2组、3组。除对照组，均加入CD4⁺CD28^null T细胞，Kv1.3阻滞1组、2组、3组同时添加2 μL 0.5、1.0、2.0 mg/mL的ADWX-1。共培养48 h，测定细胞Kv1.3钾电流峰值、内皮细胞凋亡率及培养液INF-γ和TNF-α。结果对照组、DM组、DR组、DN组和DR+DN组的CD4⁺CD28^null T细胞比例依次升高，任意2组间的差异均有统计学意义(均P＜0.05)。与对照组相比，CD28^null T干预组细胞凋亡率、INF-γ、TNF-α水平升高，差异有统计学意义(均P＜0.05)；与CD28^null T干预组相比，Kv1.3阻滞1组、2组、3组的细胞凋亡率和INF-γ水平有所降低，且3组低于2组，2组低于1组，差异有统计学意义(均P＜0.05)；TNF-α水平也有所降低，且3组低于1组与2组，差异均有统计学意义(均P＜0.05)。结论阻滞Kv1.3通道能够抑制CD4⁺CD28^null T细胞对血管内皮细胞的炎性损伤，对缓解糖尿病微血管病变有积极意义。
- Kv1.3通道 /
- CD4⁺CD28^null T细胞 /
- 糖尿病 /
- 微血管损伤
Abstract: Objective To explore the feasibility of remitting diabetic microvascular damage by blocking CD4⁺CD28^null T cell viability used Kv1.3 channel blocker. Methods Total 80 cases diabetes mellitus in the Affiliated Hospital of Medical School of Ningbo University from January 2016 to December 2017 were selected, including diabetes mellitus (DM group), diabetic retinopathy (DR group), diabetic nephropathy (DN group) and diabetic retinopathy and nephropathy (DR+DN group), with 20 cases in each group. Meanwhile, 20 healthy volunteers were selected as the control group. Their CD4⁺CD28^null T cell proportion of peripheral blood was detected and extracted. Human vascular endothelial cells were cultured with 96-well plates, and divided into 5 groups: control group, CD28^null T intervention group, Kv1.3 block group 1, group 2, and group 3. In addition to the control group, CD4⁺CD28^null T cells were co-cultured with all groups. At the same time, Kv1.3 block group 1, group 2, and group 3 were interfered by 2 μL ADWX-1, with a concentration of 0.5 mg/mL, 1.0 mg/mL, and 2.0 mg/mL successively. The peak Kv1.3 potassium current, apoptosis rate of endothelial cells and concentration of INF-γ and TNF-α were measured 48 h later. Results The proportion of CD4⁺CD28^null T cells was increased successively in the order of the control group, DM group, DR group, DN group and DR+DN group, and the difference was statistically significant among the groups (all P<0.05). Compared with the control group, the endothelial cell apoptosis rate, INF-γ and TNF-α concentration of CD28^null T in the intervention group were significantly higher, compared with CD28^null T intervention group, those indexes in Kv1.3 block group 1, group 2 and group 3 was lower, and the difference was statistically significant among the different groups (all P<0.05). Conclusion Blocking Kv1.3 channel can inhibit the inflammatory damage to vascular endothelial cells induced by CD4⁺CD28^null T cells, which is of positive significance to alleviate diabetic microvascular disease.
- Kv1.3 channel /
- CD4⁺CD28^null T cell /
- Diabetes mellitus /
- Microvascular injury