Abstract: Objective To evaluate the value of Xpert MTB/RIF combined with GenoType MTBDRplus in the rapid diagnosis of MDR-TB in Quzhou. Methods Xpert MTB/RIF detection and solid culture were performed in the county level laboratory, GenoType MTBDRplus detection of rifampicin resistant sputum, gene chip identification were performed in municipal laboratory from January 2016 to 2017 December. The sensitivity, specificity,positive predictive value, negative predictive value and Kappa value of Xpert MTB/RIF combined with GenoType MTBDRplus in the rapid diagnosis of MDR-TB in Quzhou. Results When solid culture and liquid drug sensitivity as the gold standard, Xpert MTB/RIF combined with GenoType MTBDRplus in diagnosis of multi drug resistant tuberculosis sensitivity was 82.76% (48/58), the specificity was 99.50% (1 380/1 387), the positive predictive value was 87.27% (48/55) and the negative predictive value was 99.28%(1 380/1 390), Kappa was 0.84. XpertMTB/RIF combined with GenoType MTBDRplus in diagnosis of MDR-TB time was averaged 3.15 days. Xpert MTB/RIF combined with GenoType MTBDRplus detection 1 cases of MDR-TB was 5 470. Conclusion Xpert MTB/RIF and GenoType MTBDRplus have high sensitivity and specificity in the rapid diagnosis of MDR-TB. They can be used for early diagnosis and early treatment of MDR-TB.