Abstract: Objective To study the effect of pig heart anatomy combined with virtual reality simulator in arrhythmia intervention training. Methods A total of 52 arrhythmia intervention refresher doctors were selected in department of cardiology of The Affiliated Provincial Hospital of Anhui Medical University and were divided into the observation group (n=22) and control group (n=30). Doctors in observation group were trained by pig heart anatomy combined with virtual reality simulator while those in control group were taught by traditional teaching method. Theory examination and skill examination in real environment were performed after the training and achievement differences were compared between 2 groups. Results There was no statistical significance in such baseline as gender, age, certificates, working experience and years working in vascular intervention between the two groups(all P>0.05). Theory examination average score in observation group was significantly higher than that in control group (P<0.05). Skill examination average score in observation group was higher than that in control group (P<0.05). Compared with control group, the incidence rate of operative complication was lower in the observation group. Conclusion Teaching method of pig heart anatomy combined with virtual reality simulator can help arrhythmia intervention training, which is worthy of clinical promotion.