Abstract: Objective To investigate the predictive value of Ki-67, P53 and CA153 in axillary lymph node metastasis of breast cancer. Methods We selected 168 breast cancer patients admitted to Zhuozhou hospital from January 2015 to June 2018. They were divided into lymph node metastasis group (n=116) and no lymph node metastasis group (n=52) according to whether they had axillary lymph node metastasis, and the expressions of ki-67, P53 and CA153 in each group were detected. Analysis of risk factors of axillary lymph node metastasis in breast cancer was made via univariate and multivariate logistic regression. Results Univariate analysis showed that clinical stage, tumor diameter, Ki-67, P53 and CA153 positive expression were correlated with axillary lymph node metastasis of breast cancer (P<0.05), while age, BMI, clinical stage and pathological type were not correlated with axillary lymph node metastasis of breast cancer (P>0.05). Multivariate logistic regression analysis showed that clinical stage (OR=2.307, 95% CI: 1.485-5.452), tumor diameter (OR=1.725, 95% CI:1.118-2.974), Ki-67 (OR=3.320, 95% CI: 2.907-10.781), P53 (OR=1.864, 95% CI: 1.206-3.224) and CA 153 (OR=2.113, 95% CI: 1.390-4.942) positive expression were risk factors for axillary lymph node metastasis. Conclusion Clinical stage, tumor diameter, positive expression of Ki-67, P53 and CA153 are risk factors for axillary lymph node metastasis of breast cancer, which had certain value in predicting axillary lymph node metastasis.