Abstract: Objective To explore the effect of pelvic floor reconstruction in the treatment of pelvic floor disease and the inflammatory and stress reactions of patients. Methods One hundred and six patients with pelvic floor dysfunction treated in our hospital from January 2016 to July 2017 were selected, the patients were divided into observation group (n=56) and control group (n=50) according to the final operation, the observation group was treated with pelvic floor reconstruction, while the control group was treated with sacrospinous ligament fixation, the curative effect of the two groups was observed, the quality of life of the patients was evaluated by PFDI-20, and the indexes of inflammation and stress reaction were detected before and after treatment. Results POP-Q grading in the observation group and the control group at one year after treatment were significantly better than that before treatment (all P<0.05). The POP-Q grading of the observation group at one year after treatment was significantly better than that of the control group, the difference has statistical significance (P<0.05), and the proportion of POP-Q grade 0 to I was 92.86%. The PFDI-20 scores of the observation group and the control group after treatment were lower than those before treatment (all P<0.05). The PFDI-20 score of the observation group at 3 months after treatment was (13.24±3.10) points, which was significantly lower than that of the control group, the difference had statistical significance (P<0.05). There was no significant difference in cortisol (COR), norepinephrine (NE) and prostaglandin E₂ (PGE₂) between the two groups at 24 hours after treatment (all P>0.05). There was no significant difference in interleukin-6 (IL-6) and C reactive protein (CRP) between the two groups at 24 hours after treatment (all P>0.05). Conclusion Pelvic floor reconstruction has a better effect in the treatment of pelvic floor disorders, and has no significant effect on inflammation and stress response.