Abstract: Objective To study the application of pelvic floor ultrasound in female stress urinary incontinence (SUI) and evaluate the effect of pelvic floor muscle training. Methods From April 2017 to December 2017, 1 000 primipara who were examined in our hospital were selected. The diagnostic efficacy of pelvic floor ultrasound was counted. Pelvic floor muscle training program was formulated. After 6 months, all SUI patients were divided into control group and treatment group. Pelvic floor ultrasound was used to observe the pelvic floor and peri-bladder structure data of the two groups, and the therapeutic effect was compared. Results The specificity, sensitivity and accuracy of pelvic floor ultrasonography diagnosis were higher; in resting state, the urethral inclination angle and posterior angle of bladder and urethra in treatment group were smaller than those in control group, the position of bladder neck and bladder was higher than that in control group, and the formation rate of funnel in urethra was lower than that in control group (all P<0.05); under maximum Valsalva maneuver, the descending distance of bladder neck, the rotation angle of urethra and bladder urine in the incidence of funnel formation was lower in the treatment group than in the control group (all P<0.05), and the total effective rate in the treatment group was higher than that in the control group (P<0.05). Conclusion Pelvic floor ultrasound has a high diagnostic efficiency for SUI symptoms, and can clearly show the pelvic floor structure. According to the specific conditions of patients with SUI, appropriate treatment methods are selected to provide help for early diagnosis and treatment of SUI patients.