Abstract: Objective To investigate the relationship between inflammatory response, oxidative stress and blood glucose levels in children with sepsis. Methods From August 2016 to July 2017, 79 children with sepsis in our hospital were enrolled and assigned into control group and study group according to the blood glucose level. The patients in the control group were with normal blood glucose and the study group with elevated blood glucose. The level of inflammatory response factors was tested by using enzyme-linked immunosorbent assay; SOD, GSH-PX, AOPPS and CAT were tested by using automatic biochemical analyzer; ALT, GGT and TB were detected by using radioimmunoassay; T lymphocyte was measured by Flow cytometry. Results The levels of sTNF-RI, IL-13 and IL-10 in the study group were lower than those in the control group (all P<0.05). The levels of IL-8, TNF-α and IL-1β in the study group were higher than those in the control group (all P<0.05); SOD, AOPPS and CAT in the study group were higher than those in the control group (all P<0.05); the GSH-PX level in the study group was lower than that in the control group (all P<0.05); the levels of GGT and TB in the study were higher than those of the control group (all P<0.05). The levels of CD3⁺, CD4⁺ and CD8⁺ in the study group were higher than those in the control group (all P<0.05). The level of CD4⁺/CD8⁺ in the study group was lower than that in the control group (P<0.05). Conclusion The blood glucose level in children with sepsis is closely related to the occurrence of inflammatory reaction and oxidative stress, which shows a positive correlation.