Abstract: Objective To analyze whether the Protocadherin-PC gene is associated with prostate cancer metastasis, the effects on the epithelial protoplasm of prostate cancer cells were studied. Methods The effects of inhibitd Protocadherin-PC expression by siRNA on epithelial mesenchymal metaplasia of DU-145 and PC-3 cells were detected by western blotting, morphological detection and wound healing. Results The result of western blotting showed that suppression of the Protocadherin-PC expression could promote mesenchymal epithelial transition of DU-145 and PC-3 cells. Morphologic examination indicated that inhibition of Protocadherin-PC expression could induce DU-145 and PC-3 cells to be closer to androgen-dependent prostate cancer LNCaP. Wound healing assay suggested that the growth rate of DU-145 and PC-3 cells were down regulated by suppression of Protocadherin-PC expression. Conclusion Suppression of the Protocadherin-PC expression by siRNA reduces the invasive and metastasis ability of androgen-independent prostate cancer cells DU-145 and PC-3, and thus revealing another new function of Protocadherin-PC.