Abstract: Objective To explore the practice teaching methods for medical laboratory students in the department of blood transfusion, summarize experiences, perfect the management system, and improve the quality of practice teaching. Methods The medical laboratory students in the People's Hospital of Wenshan Zhuang and Miao Autonomous Prefecture of Yunnan Province from 2011 to 2017 were choose as the object of this study. The reform and practice of teaching are carried out in many aspects, such as improving work flow, enriching teaching methods, strengthening pre-job education, consolidating basic knowledge, improving practical ability, setting up initiative service and quality first consciousness, cultivating interest in scientific research and strengthening assessment. Results Continuous improvement of work flow, innovation in teaching methods, as much as possible to let the students to participate in the whole teaching activities, to create conditions to arrange the part of the intern for lectures, all those measures not only could exercise the expression ability, enhance the psychological quality, but also deepen the understanding and memory of the knowledge of blood transfusion, and improved the interest in blood transfusion work. Strengthening students' legal awareness had been lay the foundation for their future use of legal weapons to protect their work and stay away from medical disputes. Through a series of reforms and practices, students' ability of independent learning and independent thinking was reflected, and scientific research quality, quality awareness and professional level had been continuously improved. They had gained a sense of accomplishment and pride in the practice of blood transfusion department, and gradually transform passive learning into active learning. At the same time, the teachers were encouraged to strengthen their own learning and become excellent, and their teaching level has been significantly improved. They have won many outstanding achievements in theoretical teaching competitions at the teaching and research section, the hospital and the Guangdong medical university. Conclusion The practice teaching of medical laboratory students in the department of blood transfusion is helpful to improve the quality of practice teaching through continuous exploration, reform and practice, and summarizing experience. It can provide reference for relevant university and hospital, and has certain significance for promoting teaching reform.