Abstract: Objective To investigate the ability of pre-examination and triage of emergency nurses, to explore the influencing factors, and to provide reference for the training of nurses in emergency department. Methods A random sample method was used to select 177 emergency pre-examination nurses from the top three hospitals in our city. The pre-examination and triage ability were investigated, and the influencing factors affecting the pre-examination ability of emergency nurses were analyzed. Results The total score of 177 emergency nurses was 101 points, the maximum value was 214 points, and the average score were (157.80±26.42) points. Cognitive behavior, experience and technical confidence, intuition and critical thinking were scored (35.09±7.11), (47.37±7.29), (27.54±6.43) and (47.78±8.50), respectively. Univariate analysis showed that the length of emergency work, the length of pre-screening and triage work, educational background, professional title, whether to obtain emergency related certificates, whether to receive formal training in emergency triage. Participation in case discussion and examination interval were the main factors influencing the ability of pre-examination and triage of emergency nurses (P<0.05). Logistic multivariate regression analysis showed that the pre-examination work years, academic qualifications, professional titles, whether the formal training was conducted through emergency triage, whether the participation in the case discussion and the assessment interval were independent influencing factors of the pre-examination ability of the emergency nurses (all P<0.05). Conclusion The pre-examination ability of emergency nurses is generally at a general level, especially for nurses with short working hours, low academic qualifications and professional titles, formal training without emergency triage, non-participating case discussion and long interval between assessments.