Abstract: Objective To study the effect of ultra-early comprehensive intervention on early intellectual and physical development of late premature infants, and to provide reference for effective clinical intervention. Methods A total of 116 late preterm infants born in Changning Maternal and Infant Health Care Hospital of Shanghai from April 2017 to April 2018 and managed in the follow-up clinic of premature infants, which had poor repertoire GMs were randomly divided into intervention group(56 cases) and control group(60 cases) used by random number table method. In addition to the normal follow-up in Preterm Infant Clinic, the intervention group was given three comprehensive interventions(visual music therapy, sensory integration training and traditional Chinese massage) within 3 months of corrected gestational age for one course of treatment. The Gesell Developmental Scale(GDS) was used for late preterm infants at the corrected age of 6 months, and their weight, length and head circumference were simultaneously measured, and to compare the intellectual and physical development of the two groups. Results The gross motor, adaptability and social competence scores of the intervention group at the corrected age of 6 months were(99.64±8.50) points,(94.93±7.46) points and(96.59±6.34) points, which were significantly higher than(94.42±8.29) points,(89.67±10.92) points and(91.65±7.44) points of the control group, with statistically significant differences(all P<0.05). In fine motor and language areas, the scores of intervention group were(87.66±12.01) points and(91.41±8.72) points, while the control group were(83.97±9.42) points and(90.53±11.46) points, there was no significant difference between the two groups(all P>0.05). There was also no significant difference in weight, length and head circumference between the intervention group and the control group when correcting gestational age of 6 months(all P>0.05). Conclusion Ultra-early comprehensive intervention can promote early intellectual development of late preterm infants, but it has no obvious effect on early physical development.