Abstract: Objective To understand and analyze the effect of assistant general practitioner training in Yunnan Province, and try to further improve the teacher training mode, and provide feasible suggestions for accelerating the construction of the teaching staff team of Yunnan Province assistant general practitioner training, in order to enhance the nation assistant general practitioner training teacher training results to provide basis and reference. Methods Questionnaires and interviews were conducted to conduct a questionnaire survey and on-site interviews with 139 participants who participated in the first assistant general practitioner training course in Yunnan Province in 2018. Results The percent of the participants in the training were satisfied with the overall evaluation of the teacher training class was 91.13%. The overall evaluation of the 6.45% of the participants in the training course was general. Only 2.42% of the participants participated in the training were dissatisfied. Personal interest and unit support mainly affect the enthusiasm of the participants to participate in the training. After the participants participate in the training, the knowledge and skills that still need to be increased are in turn the teaching ability, organizational management ability and the basic concept of general medicine. Conclusion Regular training of assistant general practitioner teachers training can effectively improve the ability of teachers to teach, but there are still some factors that affect the effectiveness of teacher training. Understand the factors affecting the effectiveness of teacher training, combine the actual situation and analyze the reason, supplement and improve the selection criteria for teacher training; increase the emphasis on teacher training, mobilize the enthusiasm of assistant general teachers to participate in training; further enrich the mode and content of teacher training classes, to ensure teacher training quality and to improve the teaching level of training teachers.